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. 2024 Nov 5;14(21):e5092. doi: 10.21769/BioProtoc.5092

Table 10. PCR program for identification of positive colony.

Temperature Time Cycle number
94 °C 3 min 1 cycle
94 °C 15 s 23 cycles
55 °C 15 s
72 °C 30 s
72 °C 5 min 1 cycle