Table 10. PCR program for identification of positive colony.
Temperature | Time | Cycle number |
---|---|---|
94 °C | 3 min | 1 cycle |
94 °C | 15 s | 23 cycles |
55 °C | 15 s | |
72 °C | 30 s | |
72 °C | 5 min | 1 cycle |
Temperature | Time | Cycle number |
---|---|---|
94 °C | 3 min | 1 cycle |
94 °C | 15 s | 23 cycles |
55 °C | 15 s | |
72 °C | 30 s | |
72 °C | 5 min | 1 cycle |