Table 11. Reaction for Golden Gate assembly.
| Reagent | Volume |
|---|---|
| 10× Cutsmart buffer | 2 μL |
| 10× T4 ligase buffer | 2 μL |
| pU6X:sgRNA#X | 100 ng each type |
| pGGDestISceIEG-XsgRNA | 50 ng |
| T4 DNA ligase | 1 μL |
| Bsa | 1 μL |
| H2O | To 20 μL |
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