Table 16. PCR reaction for amplification of the coding sequence of the target gene.
| Reagent | Volume |
|---|---|
| 2× Phanta Max Mixture | 25 μL |
| cDNA | 1 μL |
| Forward primer (10 μm) | 2 μL |
| Reverse primer (10 μm) | 2 μL |
| deionized H2O | 20 μL |
| Total volume | 50 μL |
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