| Reagent | Final concentration | Quantity |
|---|---|---|
| NaCl | 116 mM | 6.779 g |
| KCl | 2.9 mM | 0.216 g |
| CaCl2·2H2O | 1.8 mM | 0.265 g |
| HEPES | 5 mM | 1.192 g |
| H2O | n/a | 1,000 mL |
| Total | n/a | 1,000 mL |
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