Fig. 1. (A) Solubility and (B) deviation from the population average, charge score, and fold propensity of TgROP4 predicted through the Protein-Sol server; (C) prediction of TgROP4 sequence phosphorylation regions in terms of serine (S), threonine (T), and tyrosine (Y) through NetPhos 3.1 server; (D) graphical diagram of phosphorylation sites; and (E) prediction of TgROP4 protein transmembrane helices. Statistics and a list of the location of the predicted transmembrane helices and the predicted location of the intervening loop regions. Length: the length of the protein sequence; number of predicted TMHs: the number of predicted transmembrane helices; Exp number of AAs in TMHs: the expected number of amino acids in the transmembrane helices. If this number is >18, it is very likely to be a transmembrane protein (or have a signal peptide). Exp number, first 60 amino acids: the expected number of amino acids in transmembrane helices in the first 60 amino acids of the protein. If this number is >a few, the predicted transmembrane helix in the N-term could be a signal peptide; total prob of N-in: the total probability that the N-term is on the cytoplasmic side of the membrane. (F, G) Graphical representations of the secondary structure prediction of TgROP4 prepared by GOR IV (F) and SOPMA online servers (G) (e=extended strand, h=helix, and c=coil).