Fig. 1.

Paired PCA plots including 95% confidence ellipses for each stream showing significant separation between New Zealand (NZ) and Scotland (SC) sites for each ionization mode. PC 1 and PC 2 scores for each, explain the observed variance between clusters. a = C18 pos, b = C18 neg, c = HILIC pos and d = HILIC neg. PERMANOVA (999 permutations) provides verification of the robustness of the cluster formations in all four analyses. C18 pos, F = 27.17, p = 0.001; C18 neg F = 14.77, p = 0.001; HILIC pos F = 38.95, p = 0.001 and HILIC neg F = 38.73, p = 0.001.ara>