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. 2024 Nov 7;14:27133. doi: 10.1038/s41598-024-78602-0

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© The Author(s) 2024

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Fig. 6 — Eosinophil depletion increases collagen deposition shown by immunohistochemistry and second harmonic generation images. Slides were stained with PDGFRα (red), DAPI (blue) and α-SMA (green) after which they were imaged on a LSM780 NLO confocal microscope that was modified to also enable forward second harmonic generation (fSHG, yellow) imaging. The three immunohistochemical channels were combined with the fSHG channel, which revealed an increased collagen deposition in the anti-CCR3 injected mice (bottom row) in comparison to the isotype injected mice (top row). SHG = second harmonics generation.