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. 2024 Oct 25;15:1475179. doi: 10.3389/fimmu.2024.1475179

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Copyright © 2024 Yamada, Nagafuchi, Yamada, Suzuki, Natsumoto, Ota, Takazawa, Hatano, Kono, Harada, Shoda, Okamura, Kosaki and Fujio

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peripheral blood immune-cell RNA-seq analysis of the case. (A) Flowchart illustrating the process of cell sorting, RNA sequencing, and analysis. (B) Number of differentially-expressed genes. (C) Reactome pathway analysis of differentially expression genes. Pt, patient; HC, healthy controls; FACS, fluorescence assisted cell sorting; MACS, magnetic-activated cell sorting, Naïve CD4, Naïve CD4 T cells; Mem CD4, Memory CD4 T cells; Th1, T helper 1 cells; Th2, T helper 2 cells; Th17, T helper 17 cells; Tfh, T follicular helper cells; Fr. I nTreg, Fraction I naïve regulatory T cells; Fr. II eTreg, Fraction II effector regulatory T cells; Fr. III T, Fraction III non-regulatory T cells; Naïve CD8, Naïve CD8 T cells; CM CD8, Central memory CD8 T cells; EM CD8, Effector memory CD8 T cells; TEMRA CD8, CD8+ T effector memory CD45RA+ cells; NK,Natural killer cells; Naïve B, Naïve B cells; USM B, Unswitched memory B cells; SM B, Switched memory B cells; DN B, Double negative B cells; Plasmablast, Plasmablasts; CL Mono, Classical monocytes; CD16p Mono, CD16 positive monocytes, Int Mono, Intermediate monocytes; NC Mono, Non-classical monocytes; mDC, Myeloid dendritic cells; pDC, Plasmacytoid dendritic cells.