Fig. 2.

In solution complex formation of MtDUT^WTwith Stl^WTor Stl^NT. (a) Size exclusion chromatography curves showing the chromatography of individual Stl^WT, Stl^NT and MtDUT^WT and protein complexes. The curves showing chromatography of Stl^WT and Stl^NT proteins are presented in peach colour, as solid and dashed lines, with 14.5 ml and 16.9 ml peak elution volumes, respectively. The MtDUT^WT curve is shown as a solid light green line with 14.8 ml peak volume. The curves corresponding to the protein-protein complexes are shown as solid and dashed dark gray lines, with 11.1 ml and 13.0 ml for the MtDUT^WT–Stl^WT complex and 13.2 ml elution peak volume for the MtDUT^WT–Stl^NT complex. The symbols showing the individual proteins, and the protein-protein complexes are similar to the ones presented in Fig. 1. (b) ESI-MS spectra of MtDUT^WT-Stl^WT mixture. The peak series of 4569 (with 25 + charge), 4761 (24+), 4968 (23+), 5194 (22+), 5442 (21+), 5717 (20+), 6017 (19+), 6364 (18+) indicate different charged states of a 114.200(± 0.080) kDa particle that corresponds to a complex with 3:2 binding stoichiometry. (c) Mass photometry histograms showing complex formation between MtDUT^WT and Stl^WT or Stl^NT. The curves and figures corresponding to the individual proteins or the complex are similar to panel (a). (d-e) Native gel electrophoresis showing complex formation between MtDUT^WT and Stl^WT (d), MtDUT^WT and Stl^NT (e) at different mixing ratios.