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Complex formation kinetic parameters of StlWT and StlNT with MtDUTWT and MtDUTΔloop.
| Ligand | Analyte | KD* (pM) |
kon (M− 1s− 1) (105) |
koff (s− 1) (10− 5) |
|---|---|---|---|---|
| StlWT | MtDUTWT | < 1 | 2.70 ± 0.01 | < 0.01 ± 0.01 |
| StlWT | MtDUTΔloop | 33 ± 1 | 18.16 ± 0.01 | 5.99 ± 0.14 |
| StlNT | MtDUTWT | 190 ± 1 | 5.42 ± 0.01 | 10.28 ± 0.04 |
| StlNT | MtDUTΔloop | Heterogenous binding | ||
*χ2 value was below 0.3 in all cases.
