Skip to main content
. 2024 Oct 23;146(44):30565–30572. doi: 10.1021/jacs.4c12035

Figure 2.

Figure 2

Synthesis of a BODIPY fluorogen-activator pair for chemokine ligation and live-cell imaging. (a) Synthesis of the fluorogenic BODIPY 5 bearing a methyltetrazine quencher and a PEG-maleimide handle for bioconjugation. (b) Structures of endo-9-hydroxymethyl-bicyclo[6.1.0]non-4-yne (BCN) and trans-cyclooctenol (TCO) as tetrazine-reactive groups. (c) Time-dependent fluorescence emission of tetrazine-BODIPY 4 (10 μM, λexc: 480 nm, λem: 510 nm) before and after reaction with BCN or TCO (1 mM) (n = 3). (d) Time-course fluorescence microscopy images of MCF-7 cells after incubation with compound 4 (10 μM, green) and addition of BCN (100 μM). Movie S1. Cell nuclei were counterstained with DRAQ5 (5 μM, red). Scale bar: 10 μm.