FIGURE 2.

Heterozygous deletion of Smpd3 expression does not result in altered EV levels in biofluids. (a) Schematic representation of Smpd3 ^+/+ and Smpd3 ^+/− mice. (b–k) Quantification of CD9⁺ EVs in CSF (b–f) and plasma (g–k) from Smpd3 ^+/+ (n = 3–4) and Smpd3 ^+/− (n = 3–6) mice with the ExoView R100 platform, showing the total (b, g), CD81⁺ (c, h), CD63⁺ (d, i), CD9⁺ (e, j) and label‐free scatter⁺ (50–200 nm) (f, k) EVs, captured on the CD9 spot. Data are represented as means ± SEM. Statistical analyses were performed by unpaired t‐testing or Mann–Whitney testing (ns, not significant). CSF, cerebrospinal fluid; EV, extracellular vesicle; Smpd3, sphingomyelin phosphodiesterase 3.