TABLE 1.

Sequences of the forward and reverse primers used for deflox PCR, RT‐qPCR and genotyping PCR.

Deflox PCR
Gene	Forward (5′–3′)	Reverse (5′–3′)
Smpd3	CACCCTCAGATCCTAACGCA	GTCGCTCCCACTCTACAGAT
Alix	CAGTTGATTAGATGTGTGAACTTAG	GGATACTCACCATCATGGACAAACCACTCTATGCAAGCTTCAAAACTGACTGAG

RT‐qPCR
Gene	Forward (5′–3′)	Reverse (5′–3′)
Rpl	CCTGCTGCTCTCAAGGTT	TGGTTGTCACTGCCTCGTACTT
Hprt	AGTGTTGGATACAGGCCAGAC	CGTGATTCAAATCCCTGAAGT
Ubc	AGGTCAAACAGGAAGACAGACGTA	TCACACCCAAGAACAAGCACA
Gapdh	TGAAGCAGGCATCTGAGGG	CGAAGGTGGAAGAGTGGGAG
Smpd1	TGGGACTCCTTTGGATGGG	CGGCGCTATGGCACTGAAT
Smpd2	TGGGACATCCCCTACCTGAG	TAGGTGAGCGATAGCCTTTGC
Smpd3	ACACGACCCCCTTTCCTAATA	GGCGCTTCTCATAGGTGGTG
Alix	TAGTGTTTGCACGGAAGACAG	GGGAGGACTGATAGGCTGGA
Tsg101	TCTAACCGTCCGTCAAACTGT	TTGTACCAGTGAGGTTCACCA
Stam1	ACCCCTTCGACCAGGATGTT	CCACAGTTTGATACACATGCTCC

Genotyping PCR
	Forward (5′–3′)	Reverse (5′–3′)
Smpd3+/−	TATGAAGTGAAGCCAGGGG	CCCACACAACCCCAAGAAACGTGGCCTATCACTGTTAC
Smpd3fl/fl	CACCCTCAGATCCTAACGCA	TGTGACTCTGGATGATCAGT