Figure 1.

Overview of the experimental workflow. From left to right, workflow begins with 9-month-old mice fed either a WD or CD for 7 weeks, resulting in increased or normal weight gain, respectively. Cryosectioned tissues were thaw mounted onto ITO glass slides and subsequently coated with the MALDI matrix DAN. Data acquisition was performed on a MALDI mass spectrometer with data analysis completed through SCiLS software.