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. 2024 Nov 7;16(1):2424911. doi: 10.1080/19490976.2024.2424911

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — (a) Characterization of NADPH-dependent 5β-dihydroprogesterone reductase (ApmAB) purified from C. innocuum strain RGG8. (b) Phylogenetic analysis of 356 Etfαβ sequences, (including 206 ApmAB). The phylogenetic relationship was determined using Maximum-likelihood method and the bootstrap values were calculated based on 500 replicates. (c) Expression of apmA induced by progesterone (Ci) and glucose (Cii). All three cyclic compounds had a working concentration of 0.3 mm. The final DMSO concentration in the assay mixtures was 0.3% (v/v). Abbreviations: PGT, progesterone.