Table 3.

Isolation and characterization of Clostridium species from the fecal samples of patient 1.

Bacterial Identities		Progesterone transformation		progesterone metabolic genes		Antibiotics susceptibility#
Clostridium species	Strains	Epipregnanolone production	Pregnanolone production	apmA (LMAICMKE_01819)	apmB (LMAICMKE_01820)	Metronidazole (broth) (MIC; μg/mL)	Metronidazole (agar) (MIC; μg/mL)	Vancomycin (broth) (MIC; μg/mL)
C. innocuum	RGG8	+++	+	+	+	1.25	0.30	>2.50
	RBW1-1	++	+	+	+	1.25	0.30	>2.50
	BT6	++	+	+	+	1.25	0.30	>2.50
	BP16	++	+	+	+	1.25	0.30	>2.50
	RBW2-1	++	+	+	+	1.25	0.30	>2.50
C. difficile*	BCRC17678	-	-	-	-	1.25	0.25	2.50
	BCRC17702	-	-	-	-	1.25	0.25	2.50
	BCRC17900	-	-	-	-	1.25	0.25	2.50
	BCRC80997	-	-	-	-	1.25	0.25	2.50
	BCRC80998	-	-	-	-	1.25	0.25	1.25
	BCRC80999	-	-	-	-	0.63	0.25	1.25
C. butyricum	M3	-	-	-	-	0.31	0.10	1.25
	C14-1	-	-	-	-	0.31	0.10	1.25
	C14-4	-	-	-	-	0.31	0.10	1.25
	C14-50	-	-	-	-	0.31	0.10	1.25
	C14-51	-	-	-	-	0.31	0.10	1.25
C. scindens	L1-10	-	-	-	-	0.15	0.70	2.50
	L1-28	-	-	-	-	0.15	0.70	2.50
	L1-33	-	-	-	-	0.15	0.70	2.50
C. subterminale	L2B4-2a	-	-	-	-	>2.50	>2.00	1.25
C. tertium	L2D7-4	-	-	-	-	>2.50	>2.00	1.25
C. acetobutylicum	L3D7-7	-	-	-	-	0.15	0.10	1.25

*Strains of C. difficile were obtained from Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan, because the gut microbiota of Patient 1 did not contain C. difficile.

^#The antibiotics were tested in the dose ranging from 0.02 to 2.50 μg/mL (for bacterial growth in broth) and 0.02 to 2.00 μg/mL (for bacterial growth on agar).

+++Over 80% of the substrate was transformed into corresponding product. ++ Over 50% (but less than 80%) of the substrate was transformed into corresponding products. +, Over 10% (but less than 50%) of the substrate was transformed into corresponding products.