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. 2024 Oct 23;16(21):3565. doi: 10.3390/cancers16213565

Figure 5.

Figure 5

Panobinostat induces G1 cell cycle arrest in Ewing Sarcoma. (A,B) Cells stably expressing the Incucyte® Cell Cycle Lentivirus Reagent were treated with Panobinostat (vehicle control, approximate ½ IC50, or approximate IC50) for 24 h and images were collected on an Incucyte S3 live cell imaging system. (A) Images were quantified using the Incucyte® Basic Anlayzer software and the percentage of cells, out of all the cells counted within that well, in each phase are shown. Two-way ANOVA with Dunnett’s multiple comparisons test indicates significance: ns: no significance, ** p < 0.01, and **** p < 0.0001. The data are the average of 3 experiments and the error bars are SEM. (B) The representative images of the indicated timepoint and Panobinostat concentrations are shown for each cell line tested. The cells in the G1 phase are shown in red, the cells in G1/S are shown in yellow, and the cells in S/G2/M are shown in green.