Table 1.

Primers and PCR conditions for the detection of the investigated resistance genes.

Target Gene	Primers Sequence (5′-3′)	Annealing Temp. (°C)	Amplicons Size (bp)	References
bla NDM	GGTTTGGCGATCTGGTTTTC CGGAATGGCTCATCACGATC	52	621	[27]
bla KPC	CGTCTAGTTCTGCTGTCTTG CTTGTCATCCTTGTTAGGCG	52	798	[27]
bla OXA-48	GCGTGGTTAAGGATGAACAC CATCAAGTTCAACCCAACCG	52	438	[27]
bla IMP	GGAATAGAGTGGCTTAAYTCTC GGTTTAAYAAAACAACCACC	52	232	[27]
blaVIM	GATGGTGTTTGGTCGCATA CGAATGCGCAGCACCAG	52	390	[27]
bla TEM	GCACGAGTGGGTTACATCGA GGTCCTCCGATCGTTGTCAG	60	310	[28]
blaSHV	TTCGCCTGTGTATTATCTCCCTG TTAGCGTTGCCAGTGYTCG	50	854	[29]
blaCTX-M	ATGTGCAGYACCAGTAARGTKATGGC TGGGTRAARTARGTSACCAGAAYCAGCGG	60	593	[29]
blaCMY-1	GTGGTGGATGCCAGCATCC GGTCGAGCCGGTCTTGTTGAA	58	915	[29]
blaCMY-2	GCACTTAGCCACCTATACGGCAG GCTTTTCAAGAATGCGCCAGG	58	758	[29]