Table 2.

Primers and PCR conditions for the detection of virulence genes.

Pathotype	Target Gene	Primers Sequence (5′-3′)	Annealing Temp. (°C)	Amplicon Size (bp)	Reference
STEC/ EHEC	stx1	ATAAATCGCCATTCGTTGACTAC AGAACGCCCACTGAGATCATC	60	180	[30]
	stx2	GGCACTGTCTGAAACTGCTCC TCGCCAGTTATCTGACATTCTG	60	255	[30]
	hylA	GCATCATCAAGCGTACGTTCC AATGAGCCAAGCTGGTTAAGCT	60	534	[30]
EHEC/ EPEC	eaeA	GACCCGGCACAAGCATAAGC CCACCTGCAGCAACAAGAGG	60	384	[30]
EPEC	escV	ATTCTGGCTCTCTTCTTCTTTATGGCTG CGTCCCCTTTTACAAACTTCATCGC	53	544	[4]
	bfpB	GACACCTCATTGCTGAAGTCG CCAGAACACCTCCGTTATGC	53	910	[4]
	ent	TGGGCTAAAAGAAGACACACTG CAAGCATCCTGATTATCTCACC	53	629	[4]
ETEC	LT	GAACAGGAGGTTTCTGCGTTAGGTG CTTTCAATGGCTTTTTTTTGGGAGTC	53	655	[4]
	STIa	CCTCTTTTAGYCAGACARCTGAATCASTTG CAGGCAGGATTACAACAAAGTTCACAG	53	157	[4]
	STI	TGTCTTTTTCACCTTTCGCTC CGGTACAAGCAGGATTACAACAC	53	171	[4]
EIEC	invE	CGATAGATGGCGAGAAATTATATCCCG CGATCAAGAATCCCTAACAGAAGAATCAC	53	766	[4]
EAEC	astA	TGCCATCAACACAGTATATCCG ACGGCTTTGTAGTCCTTCCAT	53	102	[4]
	aggR	ACGCAGAGTTGCCTGATAAAG AATACAGAATCGTCAGCATCAGC	53	400	[4]
	pic	AGCCGTTTCCGCAGAAGCC AAATGTCAGTGAACCGACGATTGG	53	1111	[4]
NTEC	CNF1	GGGGGAAGTACAGAAGAATTA TTGCCGTCCACTCTCTCACCAGT	55	1111	[31]
	CNF2	TATCATACGGCAGGAGGAAGCACC GTCACAATAGACAATAATTTTCCG	55	1240	[31]
	cdt-I	CAATAGTCGCCCACAGGA ATAATCAAGAACACCACCAC	56	411	[31]
	cdt-II	GAAAATAAATGGAATATAAATGTCCG TTTGTGTTGCCGCCGCTGGTGAAA	56	556	[31]
	cdt-III	GAAAATAAATGGAATATAAATGTCCG TTTGTGTCGGTGCAGCAGGGAAAA	56	555	[31]
	cdt-IV	CCTGATGGTTCAGGAGGCTGGTTC TTGCTCCAGAATCTATACCT	56	350	[31]