Figure 3.

Long-term CBD inhalation improved cognitive function and ameliorated the AD symptoms. (a) Novel Object Recognition (NOR) test showing inhaled CBD improved cognitive function in 5xFAD mice compared to placebo-treated mice. Representative heat maps showing spatial distribution of mouse exploration in the testing arena, with warmer colors (e.g., red) indicating areas where the mouse spent more time exploring, and cooler colors (e.g., blue) indicating areas of less exploration. The familiar object in the right lower area of the field was replaced with a novel object. (b) Bar graphs depicting the quantification of NOR results. (c) Open Field testing suggests that CBD inhalation could ameliorate the cognitive function in 5xFAD mice. The figure shows a heat map generated from an Open Field test (OF) in 5xFAD mice treated with inhaled CBD and/or placebo. (d) OF results are quantified and displayed as bar graphs, suggesting improved cognitive function in AD mice treated by CBD inhalation compared to mice treated by placebo (** p ≤ 0.01).