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. 2024 Oct 22;25(21):11366. doi: 10.3390/ijms252111366

Figure 5.

Figure 5

PhAs-LHP inhibited MiaPaCa-2 proliferation via LHP-mediated macropinocytosis uptake. Uptake of 5FAM-labeled LHP peptide into live MiaPaCa-2 human cells. (a) Typical confocal image of 5FAM-LHP (green) in live cells counterstained with Hoechst 33342 (blue) following incubation (1 h, 37 °C). Image taken using a Leica SP8 microscope at 63× magnification where scale bar = 50 µm. (b) Comparison of the uptake of 5FAM-LHP in live MiaPaCa-2 cells following 1 h incubation in the absence or presence of the macropinocytosis inhibitor amiloride (25 µM, 1 h co-treatment or 16 h pre-treatment). Uptake is presented as the mean total particle area/total cell area. Error bars represent the standard deviation from the mean (n = 8–15). (c) Comparison of the IC50 values obtained from MTT assays of MiaPaCa-2 cells following 24 h treatment with ATO, PAO, or PhAs-LHP alone or in the presence of amiloride (25 µM) for 24 h. Error bars represent the standard deviation from the mean (n = 3). Statistical significance is indicated by **, p < 0.01, or ****, p < 0.0001, ns, not significant.