Figure 3.

Myeloid-specific XBP1 deficiency decreases inflammatory cell infiltration and proinflammatory mediators in ischemic livers. The IRI model mice were subjected to 90-minute ischemia, followed by 6-hour reperfusion. (A, B) Macrophage infiltration was analyzed by immunohistologic staining with antibodies against F4/80 or CD11b; F4/80⁺ or CD11b⁺ cells were quantitated by counting numbers of positive cells/area, with the scale of 200 μm. (C) Neutrophil infiltration was analyzed by immunohistologic staining with antibodies against Ly6G; Ly6G⁺ cells were quantitated by counting numbers of positive cells/area, with the scale of 200 μm. (D) Detection of TNF-α, IL1β, IL6, iNOS, and IL10 in ischemic livers using qRT-PCR. (E) Detection of TNF-α, IL6, and IL10 in serum by enzyme-linked immunosorbent assay. n = 6, ∗P < .05 by Student t test.