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. 2024 Oct 29;25(21):11596. doi: 10.3390/ijms252111596

Figure 5.

Figure 5

Content of the subunits of five respiratory chain complexes (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1, and CV-vATP5A) in rat heart mitochondria from the experimental groups. (A) Representative Western blot of typical oxidative phosphorylation (OXPHOS) subunits of complexes I–V in the mitochondria isolated from the organs of experimental animals. The analysis was performed using the Total OXPHOS Rodent WB Antibody Cocktail and anti-VDAC1 antibodies (Abcam, #ab110413 and #ab15895, Cambridge, UK; 10 μg of mitochondrial protein per lane). PC–positive control (Abcam, #ab110341, Cambridge, UK). (B) Densitometric analysis of the level of the OXPHOS subunits in the cardiac mitochondria of experimental animals. Data are expressed as mean values ± standard errors. Control values are taken as 100%. Significant differences tested with a one-way analysis of variance and Tukey’s post hoc test. * p < 0.05.