Figure 4.

Manuka treatment decreased cell viability. The indicated cells were plated into 24-well plates, serum-starved overnight, and treated for 24 h with the indicated concentrations of manuka essential oil. An MTT assay was then performed to determine cell viability. Controls were left untreated (“control”) or were treated with ethanol (vehicle for oil dilution) (“EtOH”). (A) CUA-4 fibroblasts. * results were the average of 8 independent experiments and were found to be significant using ANOVA (α = 0.005). (B) HT-1080 fibrosarcoma cells. * results were the average of 10 independent experiments and were found to be significant using ANOVA (α = 0.05).