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Differential expression of mitogen-activated protein kinase phosphatases (MKPs). HT-1080 fibrosarcoma cells were plated into 100 mm plates, serum-starved overnight, and treated for 4 h with 1000 μg/mL of manuka essential oil. Controls were left untreated. Western blot analysis was performed using anti-MKP1 (representative of two experiments) or anti-MKP2 antibodies (representative of three experiments) as indicated.
