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. 2024 Nov 1;16(21):3758. doi: 10.3390/nu16213758

Figure 7.

Figure 7

Capsaicin (CAPS) impairs AKT and ERK1/2 activation in malignant mesothelioma (MM) cells. Levels of total and phosphorylated AKT and ERK1/2 were evaluated by Western blotting in NCI-H2052 (a), MSTO-211H (b) and NCI-H28 (c) cells serum-starved for 5 h and then treated with 1% fetal bovine serum medium containing DMSO alone (DMSO) or CAPS at concentrations of 200 µM (CAPS 200) or 250 µM (CAPS 250) for 15′, 30′ and 1 h. Representative Western blotting images of two independent experiments (n = 2) for phosphoAKT (pAKT), total AKT (AKT), phosphoERK1/2 (pERK1/2), total ERK1/2 (ERK1/2) and GAPDH are shown. Densitometric analysis of phospho AKT/total AKT ratio and phospho ERK1/2/total ERK1/2 ratio. The values are expressed as mean ± standard deviation. Statistical differences were evaluated by one-way analysis of variance (ANOVA) (* p-value < 0.05).