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Chromatographs of metformin and the respective metformin D6 peaks, using HILIC chromatographic separation in a Waters Xevo TQXS. Shown are a system suitability test (SST), which was in solution, CAL A with blank matrix and real samples from the HEK293-Flp-in-hOCT1, HEK293-hOCT2 and HEK293-hOCT3 cell lines. The addition of the matrix negatively affected the peak shape and width achieved in solution. Furthermore, there were differences in peak shape among cell lines.
