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. 2024 Oct 26;24(21):6875. doi: 10.3390/s24216875

Figure 2.

Figure 2

Spike-dependent HRCA and LAMP. (A) Colorimetric switch of EY615 aptamer duplicate assays in HRCA (40 min) or LAMP context (30 min) with or without Spike protein. (B) Average ratios of absorption intensities at 430 nm versus 560 nm for the IAs. (C) LAMP and HRCA product migration on 3% agarose gel. Lane 9: 100 bp ladder (Froggabio). (D) LAMP assays with EY615 incubated with control proteins, cellulase B (CellB) and p53, to assess if the effect observed with Spike is obtained with a random protein, negative control assay with the long-version aptamer (EY615lv) unable to form the dumbbell-like shape necessary for the HRCA and LAMP triggering, and positive control assay with a reinforced dumbbell-like structure forming sequence (EY615rdl). (E) Summary table of the different pathways leading to the observed outcomes in HRCA and LAMP processes. HRCA or LAMP activation is represented by green upward arrows, while inhibition is shown by red downward arrows. Gray arrows symbolize the consequential effect between events. The table shows also the assays corresponding to the combination or succession of events depicted and the last column shows the expected colorimetric results.