Figure 2.

An intact ABA response is required for anthracene to inhibit gemmae germination. Dormant gemmae were placed on 0.5× Gamborg medium supplemented with 0 (Mock) or 1 μM ABA. The gemmae were collected from plants grown under control conditions (Mock) or exposed to 100 μM anthracene (Anth). They were plated on 0.5× Gamborg medium and grown under long-day conditions (80–100 μmol m⁻² s⁻¹) at 22 °C. Rhizoid emergence (germination) was scored at regular intervals of 8 h after plating. Each data point represents the average of three plates, with error bars indicating SE of proportions (n = 3). Each plate has an average of 15 plants. A one-way ANOVA was performed to assess the effect of treatment on the frequency of germination at the 8 h time point. Significant differences were further examined using Fisher’s LSD post hoc test (p < 0.05).