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| Age | Embryos in brood chamber | PTA concentration | Staining duration |
|---|---|---|---|
| Juvenile (instara 1–3) | no | 0.3% | 48 hrs |
| Juvenile (instara 4–7) | no | 1% | 48 hrs |
| Adult (instarb 8 and older) | yes | 3% | 72 hrs or longer (with a PTA renewal every subsequent 48 hrs) |
aOne instar is about 24 hours for juveniles.
bOne instar is about 72 hours for adults.
