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. 2024 Jul 18;25(11):4708–4727. doi: 10.1038/s44319-024-00208-4

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) Structural comparison of H3N2 polymerase in the transcription state (PDB ID:6RR7) and a FluAPol protomer from the FluAPol-NS2 hexamer complex. During transcription, the cap binding domain of PB2 and the endonuclease domain of PA should be aligned to enable the cap-snatching process. In the structure of the FluAPol-NS2 complex, binding of NS2 would cause a steric clash with the 627 domain of PB2 and thus prevents FluAPol from adopting the transcriptase conformation. (B) Dose-dependent effect of NS2 on the accumulation of viral RNAs in the transcription-defective (PA-D108A) RNP reconstitution system in HEK 293T cells. (C) Quantification of the effects of NS2 on viral RNA synthesis shown in (B). The graph shows the relative mean intensities of viral cRNA and vRNA normalized to 5S rRNA. The graph shows the mean ± s.e.m. from three biological replicates. Source data are available online for this figure.