Fig. 1.

Snx25^+/- mice show a tactile-insensitive phenotype. (a) Fine tactile sensitivity was examined with von Frey filaments whose mechanical pressure ranged from 0.008 g to 1 g. When the plantar surface of the hind paw was stimulated for 1 s with these filaments, withdrawal immediately after stimulus was considered as a positive response. The percentage of positive responses in 10 time stimulations with each filament was measured. The percentages of withdrawal (positive response) to innocuous low-pressure stimuli were plotted (WT: n = 14; Snx25^+/-: n = 18). X-axis values indicate target forces of von Frey filaments. 0.008g; p = 0.027, 0.04g; p = 0.009, 0.07g; p = 0.005, 0.16g; p = 0.005, 0.6g; p = 0.038. (b) mRNA expression levels for mechanosensory factors in DRG of Snx25^+/- mice were significantly lower than those of WT mice (WT: n = 4–8; Snx25^+/-: n = 4–8). Trpv2; p = 0.004, Trpc1; p = 0.042. *p < 0.05, **p < 0.01, n.s., not significant. Results are represented as mean ± SEM. Statistical analyses were performed using the Student’s t-test (a) or Welch’s t-test (b).