Fig. 3.

SNX25-expressing dermal macrophages are closely associated with CGRP-positive fibers. (a) Confocal images of the sciatic nerve of WT mice, stained with anti-CGRP and TrkA antibody. The left panels are a sagittal section and the right panels are a transverse section. Arrowheads denote CGRP⁺ TrkA⁺ nerves. (b) Confocal images of the DRG of WT mice, stained with anti-CGRP and TrkA antibody. The upper panels are DRGs and the lower panels are a primary DRG neurons. Arrowheads denote CGRP⁺ TrkA⁺ cells. (c) Confocal images of hind paw skin (naïve) stained for YFP (green) and CGRP (red) in TAM-treated Avil^CreER; Ai32 mice. EP, epidermis. (d) Fine tactile sensitivity was examined with von Frey filaments whose mechanical pressure ranged from 0.008 g to 1 g. The percentages of withdrawal from innocuous low-pressure stimuli were plotted (Snx25^fl/fl mice: n = 8; Snx25^Avil-cKO mice: n = 9). (e) Confocal images of hind paw skin (naïve) stained for YFP (green) and CGRP (red) in 4-OHT-treated Cx3cr1^CreER; Snx25^fl/fl; Ai32 mice. Arrowheads denote nerve-associated YFP⁺ cells. EP, epidermis. (f) Confocal images of hind paw skin (naïve) stained for YFP (green), MHC-II (white), and CGRP (red) in 4-OHT-treated Cx3cr1^CreER; Snx25^fl/fl; Ai32 mice. Arrowheads denote nerve-associated YFP⁺ MHC-II ⁺ cells. (g) Confocal images of hind paw skin (naïve) stained for YFP (green), F4/80 (white), and CGRP (red) in 4-OHT-treated Cx3cr1^CreER; Snx25^fl/fl; Ai32 mice. Arrowheads denote nerve-associated YFP⁺ F4/80 ⁺ cells. Results are represented as mean ± SEM. Statistical analyses were performed using the Student’s t-test (d).