Fig. 5.

NGF and SNX25 are expressed in dermal macrophages. (a) Scheme depicting dermal injection of clodronate liposome into the right hind paw and experimental schedule. (b) Fine tactile sensitivity was examined with von Frey filaments whose mechanical pressure ranged from 0.008 g to 1 g, in uninjected WT mice and in WT mice at 24 h after Clo lipo injection (n = 14). 0.02g; p = 0.023, 0.04g; p = 0.046, 0.07g; p = 0.005, 0.16g; p = 0.013, 0.4g; p = 1.4e-05, 0.6g; p = 0.030, 1.0g; p = 0.001. (c) Scheme depicting stretch of BMDMs. (d) Semi-quantitative analyses of NGF level in mechanically stretched BMDMs. BMDMs were exposed to mechanical stretch over 24 h. Mechanical stretch to 12.5% increased NGF protein expression in a time-dependent manner. (e) Semi-quantitative analyses of the immunoblots at 12 h (Con, n = 8; 12 h, n = 8). p = 0.13. The data are expressed as the mean ± S.E.M. *p < 0.05, **p < 0.01. Results are represented as mean ± SEM. Statistical analyses were performed using the Student’s t-test (b).