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. 2024 Nov 8;15:9667. doi: 10.1038/s41467-024-53564-z

Fig. 6. FNDC4 ameliorates cardiac I/R injury through activating HIF1α in vivo.

Fig. 6

a Heart samples with or without FNDC4 overexpression were collected 24 h after I/R surgery and subjected to unbiased transcriptome analysis, and the KEGG analysis was performed using the upregulated differentially expressed genes (DEGs) (n = 3). b Heart samples with or without FNDC4 overexpression were collected 24 h after I/R surgery and subjected to western blot (n = 6). c Heart samples with or without FNDC4 overexpression were collected 24 h after I/R surgery and subjected to immunofluorescence staining of HIF1α (green) and α-actinin (red). Arrows indicate HIF1α expression in the nuclei of cardiomyocytes (n = 6). d, e Mice with or without FNDC4 overexpression were treated with PX-478 to inhibit HIF1α, and heart samples were collected 24 h after I/R surgery for Evans blue and TTC double staining (n = 6). f Cardiac function of FNDC4-overexpressed mice with or without PX-478 treatment was analyzed by transthoracic echocardiography at the indicated time points (n = 6). Data were presented as the mean ± S.D., and analyzed using one-way ANOVA followed by Tukey post hoc test. *P < 0.0001. Source data are provided as a Source Data file.