Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 8;15(11):802. doi: 10.1038/s41419-024-07163-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — A The distribution of SNORD51 (red), WDR33 (green) and nuclear fractions (blue) of U251 and U373 cells was detected by IF and FISH. Nuclei were labeled with DAPI. Scale bars: 20 µm. B An enrichment of SNORD51 in WDR33 immunoprecipitated samples via RIP assay. Relative enrichment was measured by quantitative real-time PCR. Data are presented as the mean ± SD (n = 3, each group).^*P < 0.05 versus normal IgG group; ^**P < 0.01 versus normal IgG group. C RNA pull-down assay followed by western blot confirmed specific associations of WDR33 with biotinylated-SNORD51. D According to the RNAInter Database, the result showed that the binding sites of WDR33 existed in the 3’ UTR of ZBED6 pre-mRNA. E 3’ RACE PCR amplified full length 3’ UTR of ZBED6 followed by agarose gel electrophoresis confirmed that there was only one polyadenylation product. The sequence of product was detected by Sanger sequencing. F An enrichment of 3’UTR of ZBED6 pre-mRNA in WDR33 immunoprecipitated samples via RIP assay. Relative enrichment was measured by quantitative real-time PCR. Data are presented as the mean ± SD (n = 3, each group).^*P < 0.05 versus normal IgG group; ^**P < 0.01 versus normal IgG group. G RNA pull-down assay followed by western blot confirmed specific associations of WDR33 with biotinylated-3’UTR of ZBED6 pre-mRNA. H The reverse effect of WDR33 on SNORD51 in term of mRNA expression of ZBED6 in U251 and U373 cells. Data are presented as the mean ± SD (n = 3, each group). ^*P < 0.05 versus Control group; ^**P < 0.01 versus Control group; ^#P < 0.05 versus SNORD51(+) + WDR33( + )NC group. I Electrophoretic Mobility Shift Assay (EMSA) experiment proved that biotin-labeled 3’UTR of ZBED6 pre-mRNA probe could bind to WDR33. J EMSA experiment proved that biotin-labeled SNORD51 probe could bind to WDR33. K The biotin-labeled 3’UTR of ZBED6 pre-mRNA probe could The 3’UTR probe labeled with biotin ZBED6pre-mRNA could competitively bind WDR33 with the non-biotin labeled SNORD51 probe.