Table 3.

Kinetic parameters of OPZ metabolism by CYP2C19 variants.

CYP variants	Temperature	Vmax nmol/min/nmol P450	Km μM	Vmax/Km mL/min/nmol P450	37 °C ratio
CYP2C19.1A
	34 °C	3.58 ± 0.391*	1.66 (1.17–2.34)	2.23 ± 0.695	0.71
	37 °C	4.61 ± 0.259	1.49 (1.22–1.82)	3.15 ± 0.715	(1)
	40 °C	4.72 ± 0.374	1.61 (1.27–2.05)	2.98 ± 0.634	0.95
CYP2C19.1B
	34 °C	5.81 ± 0.793*	1.67 (1.56–1.80)	3.45 ± 0.217	0.97
	37 °C	7.27 ± 0.600	2.07 (1.77–2.43)	3.56 ± 0.737	(1)
	40 °C	8.16 ± 0.865	2.13 (1.86–2.43)	3.85 ± 0.599	1.08
CYP2C19.8
	34 °C	7.08 ± 1.72	11.1 (7.21–17.1)	0.655 ± 0.230	1.30
	37 °C	7.61 ± 1.44	15.2 (9.44– 24.5)	0.504 ± 0.267	(1)
	40 °C	7.20 ± 1.61	20.5 (12.6– 33.5)	0.373 ± 0.170	0.74
CYP2C19.10
	34 °C	5.10 ± 0.652	5.43 (3.60–8.20)	1.02 ± 0.507	0.86
	37 °C	5.83 ± 0.788	5.28 (3.36–8.31)	1.19 ± 0.595	(1)
	40 °C	6.42 ± 1.11	5.90 (5.13–6.79)	1.10 ± 0.272	0.92
CYP2C19.23
	34 °C	5.08 ± 1.10	5.50 (3.48–8.69)	0.988 ± 0.526	1.34
	37 °C	4.31 ± 2.48	6.33 (4.08–9.80)	0.735 ± 0.411	(1)
	40 °C	4.74 ± 1.15	6.29 (3.47–9.96)	0.787 ± 0.373	1.07

V_max, V_max/K_m: arithmetic mean ± S.D., K_m: geometric mean (−1S.D. ~ +1S.D.), n = 5

^*: p < 0.05 vs. 37 °C