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. 2024 Nov 6;65(13):12. doi: 10.1167/iovs.65.13.12

Figure 2.

Figure 2.

Reactive oxygen species (ROS) accumulation and mitochondrial dysfunction of the lacrimal gland in middle-aged mice. (A) Immunofluorescence staining of ROS using ROS probe (red) and DAPI (blue) in lacrimal gland tissues of control and middle-aged mice. (B) Assessment of relative fluorescence intensity of ROS staining. (C) Mitochondrial DNA (mtDNA) leakage in the lacrimal gland tissues in the different groups. (D) Immunofluorescence staining of mitochondrial membrane potential using JC-1 in lacrimal gland tissues of control and middle-aged mice. (E) Measurement of relative fluorescence intensity of JC-1 staining. (F) Representative immunofluorescence images for detecting TOMM20 (green) and DAPI (blue) of the lacrimal gland. (G) Representative immunofluorescence images for detecting LAMP-1 (red) and DAPI (blue) of the lacrimal gland. (H) Transmission electron microscopy (TEM) images of the ultrastructure of the acinar cells of the lacrimal gland in each group of mice. N, nucleus, Mt, mitochondria. (I) Measurement of mitochondrial area and perimeter on the TEM images. (J) TEM images of the ultrastructure of the secretory granules in the acinar cells of the lacrimal gland in control and middle-aged lacrimal glands. (K) Measurement of secretory granules (marked yellow) of acinar cells (marked red) on the TEM images. Scale bar = 20 µm (A, D, F, G), 1 µm (magnified 500 nm) (H, J), N = 4, ***P < 0.001.