Fig. 3.

Effect of TNO155 on phosphorylation levels of kinases in its downstream and parallel signaling pathways. (A) Western blot shows the effect of various TNO155 concentration on MEK and ERK phosphorylation levels upon 1-, 6- and 24-h treatment, in sensitive and resistant OSCC lines. (B) Phosphorylation arrays of DMSO-treated (control) and TNO155-treated OSCC lines reveal the overall effects of TNO155 on the downstream (MAPK) and other related phosphorylation signaling pathways (AKT, JAK/STAT, and TGFβ) and identify potential markers that are distinctive between sensitive and resistant lines. (C) Graphical representation of the quantified changes on the phosphorylated markers upon TNO155 treatment in the sensitive and resistant lines. Icons in the boxes represent whether the markers expression was upregulated (green upward arrow), downregulated (red downward arrow), or unchanged (yellow rightward arrow) upon TNO155 treatment. Differences in baseline expression were also indicated by the font colours of the markers, by comparing the signal intensities between the average of sensitive lines and average of resistant lines. Red font indicate that the markers have significantly higher level among sensitive lines than resistant lines; while green font indicate that the markers have significantly higher level among resistant lines. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)