FIGURE 7.

Patient‐derived primary HGSOC cell lines require further modifications to the taPIT protocol. Multiple‐time‐point taPIT dose responses of Cage cell line (A), and Powder cell line (B). Following 22‐h incubation with 250 nM BPD equivalent PIC and washing, 60 J/cm² light dose was administered at various time points. The cytotoxicity of taPIT decreased as the post‐wash incubation time increased. The time‐dependent cytotoxicity indicates the efflux of free BPD. (C) Updated taPIT dose–response of Cage cell line. The striking increase in efficacy of taPIT when the concentration of PIC was doubled suggests that the remaining free BPD was still causing cytotoxicity even when the updated taPIT protocol was followed since both concentrations of PIC were saturating concentrations. (D) Multiple‐time‐point free BPD PDT response of Powder cell line. Following 22‐hour incubation with 3.33 nM free BPD and washing, 60 J/cm² light dose was administered at various time points. This result shows that the free BPD does not kill the cells when the concentration is low, and the post‐wash incubation time is prolonged. Results are mean ± standard deviation of four replicates.