Fig. 3.

Comparison of microsatellite polymorphisms detected by high-resolution melting and capillary electrophoresis. On the left, the HRM-shifted melting normalized melting curve and the difference curves are shown. Each curve indicates the melting status for each accession. Different colored curves represent the different cluster of assignment of the accessions studied. On the right, fragment analysis profiles of fluorescence-labeled microsatellites separated by capillary electrophoresis. The number above each peak indicate the alleles sizes. a marker LE20593; b marker LEaac001