Table 2.

Forward and reverse primer sequences, annealing temperatures and alleles detected for the microsatellites used to genotype 72 tomato accessions through a capillary electrophoresis assay and high-resolution melting analysis

SSR name	Forward primer	Reverse primer	Alleles detected*	Tm (°C)
LEat008a	AAGCGCGAGCTCTCTCTGATCTC	CCACGATCTCCGCCATATGC	92, 99, 100	55 °C
LEct001b	TCCAATTTCAGTAAGGACCCCTC	CCGAAAACCTTTGCTACAGAGTAGA	98, 99, 101, 102, 107, 108	53 °C
LEta003b	GCTCTGTCCTTACAAATGATACCTCC	CAATGCTGGGACAGAAGATTTAATG	103, 104, 105, 106, 108, 108	53 °C
LEta015b	ATATGCATGGACAAATCTTGAGGG	CTCGCGCATCAAATTAATGTATCAG	101, 103, 105, 107, 109, 112	55 °C
LEaat002b	GCGAAGAAGATGAGTCTAGAGCATAG	CTCTCTCCCATGAGTTCTCCTCTTC	95, 96, 102, 102, 104, 106, 107	53 °C
LEaat007b	CAACAGCATAGTGGAGGAGG	TACATTTCTCTCTCTCCCATGAG	89, 96, 97, 98, 101, 102	53 °C
LEaac001b	AGGAAGAGCGTGAGTCTGAAC	TCCTGCGCCACTTTAGAG	106, 107	55 °C
LEctt001b	CCTCTCTTCACCTCTTTACAATTTCC	CACTGGTCATTAAGTCTACAGCC	90, 90, 91, 97, 97, 100	55 °C
LEtat002b	ACGCTTGGCTGCCTCGGA	AACTTTATTATTGCCACGTAGTCATGA	192, 195, 198,	55 °C
LE20592b	CTGTTTACTTCAAGAAGGCTG	ACTTTAACTTTATTATTGCCACG	162, 164, 165, 167, 169	53 °C
LE21085b	CATTTTATCATTTATTTGTGTCTTG	ACAAAAAAAGGTGACGATACA	96, 99, 101, 102, 116, 117, 119, 120	50 °C
LECBPE3c	CCTACAAAAACTGCCTCT	TTATATCAATACAACAACATT	115, 117	56 °C
LEDIH4REc	TTTTGTAATCATCTTGGAAAC	ATTGTGTTATGATGATATTTG	86	55 °C
LELAT59c	AACAACATTTCACAAAGTGCT	CGTCTCAATGAGACAACAAGT	67, 68, 69, 70	55 °C
LELEUZIPc	GGTGATAATTTGGGAGGTTAC	CGTAACAGGATGTGCTATAGG	99, 102, 103,	55 °C
LEPRP4c	TTCATTTCTTGCAACTACGAT	CATACTAGCAACATCAAAGGG	198, 199, 229, 230	55 °C
TC1107a	TCCATCTCTCTCTAGACCTTTCT	TTCTTAAATCCTCTCACTCA	87, 89, 94, 95	56 °C
TCIC-419d	TGAGCAACATAAATGCATGTATGGC	AGAACAACTGTAGTGGTTCCATCACC	96, 101, 103, 105, 106, 444, 445, 446	55 °C
TMS56a	GATCTCAAAGGATGAACAATAC	TCATTAGGAGATTCTTTGTATCA	121, 121, 122, 123, 124, 125, 126	55 °C
TMS59a	TGAACGGGCCTTCTGTTATC	ATCATCATTATAGTTCTTAAGTGAT	100, 100, 101, 102, 108	55 °C

a = Areshchenkova and Ganal (2002), b = He et al. (2003), c = Smulder et al. (1997), d = Bae et al. (2010)

^*Via capillary electrophoresis