Table 3.
Number of loci (N°), expected heterozygosity index (He), average heterozygosity (H.av), polymorphic index content value (PIC), effective multiple ratio (EMR), marker index (MI), discrimination power (DP), and resolving power (RP) obtained from the SSRs used in the present work via capillary electrophoresis (CE) and high-resolution melting HRM) analysis
| Marker | CE-SSR | HRM-SSR | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| N° | He | Ho | PIC | EMR | MI | DP | RP | N° | He | H.av | PIC | EMR | MI | DP | RP | |
| LE20592 | 5 | 0.378 | 0.039 | 0.306 | 1.264 | 0.387 | 0.937 | 1.917 | 5 | 0.320 | 0.033 | 0.269 | 1.000 | 0.269 | 0.961 | 0.508 |
| LE21085 | 8 | 0.285 | 0.030 | 0.244 | 1.375 | 0.336 | 0.971 | 1.250 | 7 | 0.245 | 0.025 | 0.215 | 1.000 | 0.215 | 0.980 | 0.611 |
| LEaac001 | 2 | 0.411 | 0.043 | 0.326 | 1.423 | 0.464 | 0.496 | 0.901 | 4 | 0.375 | 0.038 | 0.305 | 1.000 | 0.305 | 0.938 | 0.222 |
| LEaat002 | 7 | 0.318 | 0.033 | 0.267 | 1.389 | 0.372 | 0.961 | 2.444 | 9 | 0.198 | 0.020 | 0.178 | 1.000 | 0.178 | 0.988 | 1.889 |
| LEaat007 | 6 | 0.293 | 0.031 | 0.250 | 1.069 | 0.267 | 0.969 | 1.694 | 7 | 0.245 | 0.025 | 0.215 | 1.000 | 0.215 | 0.980 | 1.943 |
| LEat008 | 3 | 0.464 | 0.048 | 0.356 | 1.097 | 0.391 | 0.867 | 1.417 | 3 | 0.460 | 0.047 | 0.367 | 0.985 | 0.362 | 0.893 | 0.147 |
| LECBPE3# | 2 | 0.500 | 0.052 | 0.375 | 1.029 | 0.386 | 0.737 | 0.171 | – | – | – | – | – | – | – | – |
| LEct001 | 6 | 0.338 | 0.035 | 0.281 | 1.292 | 0.363 | 0.954 | 2.306 | 7 | 0.245 | 0.025 | 0.215 | 1.000 | 0.215 | 0.980 | 2.000 |
| LEctt001 | 6 | 0.440 | 0.046 | 0.343 | 1.958 | 0.672 | 0.894 | 0.306 | 4 | 0.375 | 0.038 | 0.305 | 1.000 | 0.305 | 0.938 | 0.222 |
| LEDIH4RE*# | 1 | 0 | 0 | 0 | 1 | 0.000 | 0 | 0 | – | – | – | – | – | – | – | – |
| LELAT59 | 4 | 0.465 | 0.048 | 0.357 | 2.528 | 0.902 | 0.601 | 1.111 | 4 | 0.375 | 0.038 | 0.305 | 1.000 | 0.305 | 0.938 | 1.167 |
| LELEUZIP | 3 | 0.448 | 0.047 | 0.347 | 1.014 | 0.352 | 0.887 | 0.085 | 3 | 0.460 | 0.047 | 0.366 | 0.986 | 0.361 | 0.893 | 0.139 |
| LEPRP4 | 4 | 0.425 | 0.044 | 0.335 | 2.775 | 0.929 | 0.520 | 2.451 | 6 | 0.278 | 0.028 | 0.239 | 1.000 | 0.239 | 0.973 | 1.111 |
| LEta003 | 6 | 0.313 | 0.033 | 0.264 | 1.167 | 0.308 | 0.963 | 2.333 | 5 | 0.320 | 0.033 | 0.269 | 1.000 | 0.269 | 0.960 | 1.611 |
| LEta015 | 6 | 0.340 | 0.035 | 0.283 | 1.306 | 0.369 | 0.953 | 2.611 | 13 | 0.142 | 0.014 | 0.132 | 1.000 | 0.132 | 0.994 | 2.000 |
| LEtat002 | 3 | 0.450 | 0.047 | 0.349 | 1.028 | 0.359 | 0.884 | 1.444 | 4 | 0.375 | 0.038 | 0.305 | 1.000 | 0.305 | 0.938 | 0.507 |
| TC1107# | 4 | 0.379 | 0.039 | 0.307 | 1.015 | 0.311 | 0.936 | 0.324 | – | – | – | – | – | – | – | – |
| TCIC-419 | 8 | 0.398 | 0.041 | 0.319 | 2.194 | 0.700 | 0.925 | 2.056 | 10 | 0.180 | 0.018 | 0.164 | 1.000 | 0.164 | 0.990 | 2.000 |
| TMS56 | 7 | 0.301 | 0.031 | 0.256 | 1.292 | 0.330 | 0.966 | 2.583 | 2 | 0.500 | 0.051 | 0.375 | 1.000 | 0.375 | 0.752 | 0.556 |
| TMS59 | 5 | 0.323 | 0.034 | 0.271 | 1.014 | 0.275 | 0.959 | 1.887 | 5 | 0.320 | 0.033 | 0.269 | 1.000 | 0.269 | 0.960 | 0.909 |
| Total | 96.0 | 7.27 | 0.76 | 5.84 | 27.23 | 8.47 | 16.38 | 29.29 | 98.0 | 5.412 | 0.552 | 4.491 | 16.97 | 4.480 | 16.06 | 17.54 |
| Average | 4.80 | 0.383 | 0.040 | 0.307 | 1.433 | 0.446 | 0.862 | 1.542 | 5.76 | 0.318 | 0.032 | 0.264 | 0.998 | 0.264 | 0.944 | 1.032 |
*Monomorphic
#Not suitable for HRM analysis