Skip to main content
. 2024 Nov 9;15(11):810. doi: 10.1038/s41419-024-07204-5

Fig. 1. Loss of RB and p53 bypasses AURKBi-induced cell cycle arrest.

Fig. 1

A RB+p53 wild type HT1080 and RB+p53 defective CaSki cells were treated for 3 days with and without (control) 0.2 μM AZD2811 (AZD; AURKBi) then fixed and stained for DNA. Bars = 50 μm. B RB+p53 wild type HT1080 and HCT116 cells, C RB+p53 defective C33A and CaSki cells, and D immortalised human cervical keratinocytes expressing either empty vector (pLV), HPV E6 or E7 oncogenes, were treated for 6 days with 0.2 μM AZD2811, labelled for 2 h with EdU then stained for Ki67 and EdU incorporation. Cells were imaged using high content imaging and >1500 cells/well from at least three replicate wells were analysed. The percent positive staining is shown. Mean ± SD are shown. Data was collected from at least two independent experiments. Nonparametric t-test were used to compare control and treated data. The absence of comparison indicates no significant difference (***p > 0.001, ****p > 0.0001).