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. 2024 Oct 30;4(1):ycae127. doi: 10.1093/ismeco/ycae127

Figure 4.

Figure 4

The effect of antibiotics treatment on the bacterial communities and abundance in D. gallinae. (A) Shannon and ACE indexes of D. gallinae bacterial microbiota. Student’s t test. *P < .05. (B) The LDA effect size (LEfSe) algorithm with LDA scores ≥3.5 was applied to OTU tables to identify taxa that most effectively characterize each biological class. (C) Whole profiles of the relative abundances of bacterial taxa in control and treatment groups; all OTUs that accounted for <1% were combined “other.” (D) The PCoA for bacterial community composition based on Bray–Curtis. (E) The quantification of numbers of copies of total bacterial 16S rRNA gene in control and treatment groups. (F) Numbers of bacterial 16S rRNA gene copies obtained from adult females by Bartonella A specific primers. (G) Numbers of bacterial 16S rRNA gene copies obtained from eggs by Bartonella A primers. Asterisks indicate statistical significance: *P-value <.05; **P-value <.01; ***P-value <.001 in the Student’s t test. C, control; T, OTC treatment; CE, control eggs; TE, treatment eggs.