Antibodies |
|
mouse anti-DENV NS1 |
Yao-Hong Biotechnology |
YH0023 |
mouse anti-DENV E |
Yao-Hong Biotechnology |
YH0026 |
HRP-conjugated AffiniPure goat anti-mouse IgG |
Jackson ImmunoResearch |
Cat# 115-035-003; RRID: AB_10015289
|
|
Bacterial and virus strains |
|
DV-1202 |
This paper |
GenBank: MG_599602 |
|
Chemicals, peptides, and recombinant proteins |
|
Methylcellulose |
Sigma-Aldrich |
CAS:9004-67-5 |
Isoflurane |
Panion & BF biotech inc. |
N/A |
Rompun |
Bayer Animal Health |
N/A |
Ketalar |
Pfizer |
N/A |
|
Critical commercial assays |
|
NEBuilder HiFi DNA Assembly Master Mix |
NEB |
E2621 |
Q5 Site-Directed Mutagenesis Kit |
NEB |
E0554S |
VECTOR VIP Peroxidase (HRP) substrate kit |
Vector Laboratories |
SK-4600 |
SYBR Green Master Mix |
Applied Biosystems |
Cat#4344463 |
Riboprobe In Vitro Transcription Systems kit |
Promega |
P1460 |
RNeasy mini kit |
Qiagen |
74104 |
KOD Hot Start DNA Polymerase kit |
Merck/Novagen |
71086 |
Streptavidin-Sepharose HP |
Amersham Pharmacia |
Cat#17-5113-01 |
|
Deposited data |
|
Plaque and Foci figures |
This paper |
http://www.doi.org/10.17632/ywb36s3cyc.2 |
|
Experimental models: Cell lines |
|
Human: 293T/17 cells |
ATCC |
CRL-11268 |
Monkey: Vero cells |
ATCC |
CRL-1587 |
Hamster: BHK-21 |
ATCC |
CCL-10 |
Mosquito: C6/36 cells |
ATCC |
CRL-1660 |
|
Experimental models: Organisms/strains |
|
Mouse model (interferon-α/β and -γ receptor-knockout mice) |
From National Health Research Institutes, Taiwan |
4- to 6-week-old mice, AGB6 |
Mosquito model |
From National Health Research Institutes, Taiwan |
Female, Aedes aegypti, Higgs strain |
|
Oligonucleotides |
|
Random hexamers |
Promega |
Cat# C1181 |
mFU1 sequence: 5'-TACAACATGATGGGAAAGCGAGAGAAAAA-3' |
Chien et al.62
|
N/A |
CFD2 sequence: 5'-GTGTCCCAGCCGGCGGTGTCATCAGC-3' |
Chien et al.62
|
N/A |
SNV-1073TC-F sequence: 5'-ACGACGATGGCGAAAAATAAAC-3' |
This paper |
N/A |
SNV-1073TC-R sequence: 5'-Biotin-TGTTGGTCAGCTTTGCCTCTA-3' |
This paper |
N/A |
SNV-8381CT-F sequence: 5'-CGCAACATCGGAATTGAAA-3' |
This paper |
N/A |
SNV-8381CT-R sequence: 5'-Biotin-CTTGGTCATAGTGCCATGATGT-3' |
This paper |
N/A |
SNV-8639AT-F sequence: 5'-GGACAACAGCGCGTTTTCA-3' |
This paper |
N/A |
SNV-8639AT-R sequence: 5'-Biotin-CCATTCTGCCGTGATTTTCA-3' |
This paper |
N/A |
SNP-1073TC-S sequence: 5'-ACCAACATTGGATTTTGAAC-3' |
This paper |
N/A |
SNP-8381CT-S sequence: 5'-CATCGGAATTGAAAGTG-3' |
This paper |
N/A |
SNP-8639AT-S sequence: 5'-ACAGCGCGTTTTCAA-3' |
This paper |
N/A |
|
Recombinant DNA |
|
pGEM-T Easy Vector |
Promega |
A1360 |
pCMV-HDVr-SV40pA |
From Dr. Eng Eong Ooi Lab, Duke-NUS Medical School |
N/A |
|
Software and algorithms |
|
Image J |
Schneider et al.63
|
https://imagej.net |
GraphPad Prism 9.0 |
La Jolla, CA, USA |
https://www.graphpad.com |
|
Other |
|
QuantStudio 3 Real-Time PCR System |
Applied Biosystems |
https://www.thermofisher.com/tw/zt/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/quantstudio-systems/ |
PyroMark Q24 system |
Qiagen |
https://www.qiagen.com/us/products/discovery-and-translational-research/pyrosequencing/pyromark-q24-and-advanced |