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. 2024 Oct 11;27(11):110899. doi: 10.1016/j.isci.2024.110899
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

mouse anti-DENV NS1 Yao-Hong Biotechnology YH0023
mouse anti-DENV E Yao-Hong Biotechnology YH0026
HRP-conjugated AffiniPure goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-035-003; RRID: AB_10015289

Bacterial and virus strains

DV-1202 This paper GenBank: MG_599602

Chemicals, peptides, and recombinant proteins

Methylcellulose Sigma-Aldrich CAS:9004-67-5
Isoflurane Panion & BF biotech inc. N/A
Rompun Bayer Animal Health N/A
Ketalar Pfizer N/A

Critical commercial assays

NEBuilder HiFi DNA Assembly Master Mix NEB E2621
Q5 Site-Directed Mutagenesis Kit NEB E0554S
VECTOR VIP Peroxidase (HRP) substrate kit Vector Laboratories SK-4600
SYBR Green Master Mix Applied Biosystems Cat#4344463
Riboprobe In Vitro Transcription Systems kit Promega P1460
RNeasy mini kit Qiagen 74104
KOD Hot Start DNA Polymerase kit Merck/Novagen 71086
Streptavidin-Sepharose HP Amersham Pharmacia Cat#17-5113-01

Deposited data

Plaque and Foci figures This paper http://www.doi.org/10.17632/ywb36s3cyc.2

Experimental models: Cell lines

Human: 293T/17 cells ATCC CRL-11268
Monkey: Vero cells ATCC CRL-1587
Hamster: BHK-21 ATCC CCL-10
Mosquito: C6/36 cells ATCC CRL-1660

Experimental models: Organisms/strains

Mouse model (interferon-α/β and -γ receptor-knockout mice) From National Health Research Institutes, Taiwan 4- to 6-week-old mice, AGB6
Mosquito model From National Health Research Institutes, Taiwan Female, Aedes aegypti, Higgs strain

Oligonucleotides

Random hexamers Promega Cat# C1181
mFU1 sequence: 5'-TACAACATGATGGGAAAGCGAGAGAAAAA-3' Chien et al.62 N/A
CFD2 sequence: 5'-GTGTCCCAGCCGGCGGTGTCATCAGC-3' Chien et al.62 N/A
SNV-1073TC-F sequence: 5'-ACGACGATGGCGAAAAATAAAC-3' This paper N/A
SNV-1073TC-R sequence: 5'-Biotin-TGTTGGTCAGCTTTGCCTCTA-3' This paper N/A
SNV-8381CT-F sequence: 5'-CGCAACATCGGAATTGAAA-3' This paper N/A
SNV-8381CT-R sequence: 5'-Biotin-CTTGGTCATAGTGCCATGATGT-3' This paper N/A
SNV-8639AT-F sequence: 5'-GGACAACAGCGCGTTTTCA-3' This paper N/A
SNV-8639AT-R sequence: 5'-Biotin-CCATTCTGCCGTGATTTTCA-3' This paper N/A
SNP-1073TC-S sequence: 5'-ACCAACATTGGATTTTGAAC-3' This paper N/A
SNP-8381CT-S sequence: 5'-CATCGGAATTGAAAGTG-3' This paper N/A
SNP-8639AT-S sequence: 5'-ACAGCGCGTTTTCAA-3' This paper N/A

Recombinant DNA

pGEM-T Easy Vector Promega A1360
pCMV-HDVr-SV40pA From Dr. Eng Eong Ooi Lab, Duke-NUS Medical School N/A

Software and algorithms

Image J Schneider et al.63 https://imagej.net
GraphPad Prism 9.0 La Jolla, CA, USA https://www.graphpad.com

Other

QuantStudio 3 Real-Time PCR System Applied Biosystems https://www.thermofisher.com/tw/zt/home/life-science/pcr/real-time-pcr/real-time-pcr-instruments/quantstudio-systems/
PyroMark Q24 system Qiagen https://www.qiagen.com/us/products/discovery-and-translational-research/pyrosequencing/pyromark-q24-and-advanced