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. 2024 Oct 26;10(21):e39890. doi: 10.1016/j.heliyon.2024.e39890

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© 2024 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Effects of Lactucin on FFA-induced changes in the mitochondrial membrane potential and ROS levels in HepG2 cells. (A) Detection of intracellular ROS levels using the fluorescent probe DCFH-DA. (1) Control, (2) Rosup, (3) FFA, and (4) FFA+20 μmol/L lactucin. (B) The relative fluorescence intensity corresponding to each group of ROS experiments. (C) Changes in the intracellular mitochondrial membrane potential. (5) Control, (6) CCCP, (7) FFA, and (8) FFA+20 μmol/L lactucin. (D) The relative fluorescence intensity corresponding to each group of JC-1-treated cells.