Fig. 4.

Liver injury. (a) NAFLD Activity Score (NAS) displayed the severity of MASH. (b–d) shows the different components (steatosis, inflammation, ballooning) of the NAS. (e) Fibrosis score according to Kleiner et al. (f) Immunohistological results of CD45⁺ and (g) F4/80⁺ cells, (h–j) Flow cytometry was used to further identify the immune cell types. (h) The proportion of CD11b⁺F4/80⁺ macrophages were significantly higher in SG mice compared to control and Pf mice 2 weeks after surgery. (i) T-cells were and (j) CD3⁺NK1.1⁺ cells were significantly decreased in SG mice compared to control and Pf mice 2 weeks after surgery. These effects were no longer detectable in the long-term study. (k) AST and (h) ALT levels in serum before termination of the studies. (m) Liver weight measured at explantation. Data represent mean ± SD; ∗p < 0:05, ∗∗p < 0:01 and ∗∗∗p < 0:001.