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Degree of strain (DS) and procedures.
| DS | Category | Procedure | Score |
|---|---|---|---|
| DS0 | No strain | 0 | |
| DS1 | Minor category | careful observation necessary | 1–4 |
| DS2 | Moderate category | supportive measures (additional analgesia or fluids) | 5–9 |
| DS3 | Critical category | DS2 + veterinary support | 10–19 |
| DS4 | High-grade strain | consult animal protection commissary, veterinary support necessary, termination of experiment, euthanasia | ≥20 |
