Fig. 5.

CLC-P/Gal10 is present in the tumour microenvironment and correlates with poor survival. (a) Histochemical analyses of fixed tumour biopsies from MPM patients with low and high absolute eosinophil counts (AEC). Samples were fluorescently labelled for CCR3 (APC in red) and CLC-P/Gal10 (in green) and DAPI (in blue). Images were acquired using a Zeiss 880 Airyscan Elyra confocal microscope equipped with a x63–1.4 oil immersion objective. Scale bars are 10 μm. (b) Representative image of an eosinophil with a characteristic bilobed nucleus (blue) expressing CLC-P/Gal10 (green) analysed with Imaris. (c) CLC-P/Gal10 externalized by a degranulating eosinophil (white arrow). (d) M14K cells were incubated either with culture medium (mock), 25% (v/v) SN Eos or recombinant h-CLC-P/Gal10 for 48 h. Cells were fixed, labelled for CLC-P/Gal10 (green) and stained with DAPI (blue) and CellMask (red). Images were acquired using a Zeiss 980 Airyscan Elyra confocal microscope equipped with a x63–1.4 oil immersion objective. Representative images and 3D representations of CLC-P/Gal10 interaction with MPM cells were computed with Imaris. (e) ELISA titration of CLC-P/Gal10 (in ng/mL) in pleural effusions (n = 79) and sera (n = 37) from MPM patients. (f) Overall survival analysis of patients displaying high (>123.8 ng) and low (<123.8 ng) CLC-P/Gal10 levels in MPM pleural fluids.